Journal: Tissue & cell
Article Title: Detection of the nuclear translocation of androgen receptor using quantitative and automatic cell imaging analysis.
doi: 10.1016/j.tice.2024.102631
Figure Lengend Snippet: Fig. 8. Detection of nuclear translocation of Androgen receptor with Azami green fluorescence tang after the testosterone treatment of 100 nM. H2B_0h indicates the cell images obtained from DPCs which only expresses mStrawberry-labeled H2B just after the ligand stimulation. No green fluorescence. Clone 7_0h indicates the cell images obtained from the DPC of clone 7, which expresses mStrawberry-labeled H2B and Azami green-tagged AR just after the ligand stimulation. Clone 10_0h indicates the cell images obtained from the DPC of clone 10, which expresses mStrawberry-labeled H2B and Azami green-tagged AR just after the ligand stimulation. Clone 7_1h indicates the cell images obtained from DPC of clone 7, which expresses mStrawberry-labeled H2B, and Azami green-tagged AR at 1 hour after the ligand stimulation. Clone 10_1h indicates the cell images obtained from DPC of clone 10, which expresses mStrawberry-labeled H2B and Azami green-tagged AR at 1 hour after the ligand stimulation.
Article Snippet: DPCs were cultured in a follicle DPC medium (cat. no. C-26500, PromoCell,) with a supplement pack (cat. no. C-39620, PromoCell).
Techniques: Translocation Assay, Fluorescence, Labeling